Role of Trophic Hormones in Regulation of Growth and Function of Responsive Cells

The role of the trophic hormones, Adrenocorticotropic hormone (ACTH) and Luteinizing hormone (LH) in regulating growth and function of adrenocortical cells and Leydig cells respectively, has been investigated using an immunological approach. Antisera to porcine ACTH and ovine LH were raised in rabbits and characterized for specificity, ability to neutralize rat ACTH and LH respectively. Administration of ACTH antiserum to rats for 6 to 10 days did not cause any decrease in the weight of the adrenals, but resulted in a significant decrease in serum corticosterone. Also when the adrenocortical cells isolated from normal rabbit serum (NRS) or ACTH antiserum treated rats were examined for their ability to respond to exogenous ACTH, there was a drastic decrease in the response of the latter as judged by corticosterone production. It is known that in an unilaterally adrenalectomized rat there is compensatory hypertrophy of the remaining adrenal and this growth has been shown to be due to actual increase in cell number. Administration of ACTH antiserum to unilaterally adrenalectomized rats did not in any way prevent the compensatory hypertrophy response of the remaining adrenal as judged by weight, DNA, protein or yield of adrenocortical cells.

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Authors and Affiliations

1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India A. Jagannadha Rao
2. Department of Anatomy, University of California, San Francisco, CA, 04143, USA J. A. Long
3. Department of Pathology, University of Conneticuit Health Centre, Farmington, CT, 060032, USA B. D. Gondos
4. Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada J. G. Lehoux
5. Hormone Research Laboratory, University of California, San Francisco, CA, 04143, USA J. Ramachandran